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Isolamento di vettori virali adeno-associati attraverso un protocollo cromatografico di affinità con eparina a passaggio singolo e semiautomatico

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

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Isolamento di vettori virali adeno-associati attraverso un protocollo cromatografico di affinità con eparina a passaggio singolo e semiautomatico

1,094 Views

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01:57 min

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April 05, 2024

DOI:

10.3791/66550-v

DOI:

10.3791/66550-v

•

01:57 min

April 05, 2024

•

42 Views

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^1,2,4, Carina Henriques^1,2,5, Ana C. Silva^1,2,3, Diana D. Lobo^1,2,3, Luísa Cortes^1,2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC – Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB – Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC – Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector – Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC – Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC – Microscopy Imaging Center of Coimbra – CNC,University of Coimbra

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