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Journal
/
Biotechnik
/
Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography
/
Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal
Biotechnik
Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors
Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography
DOI:
10.3791/66550-v
•
01:57 min
•
April 05, 2024
•
Miguel M. Lopes*
1,2,3
,
Sara M. Lopes*
1,2,3
,
Rafael Baganha
2,4
,
Carina Henriques
2,5
,
Ana C. Silva
2,3
,
Diana D. Lobo
2,3
,
Luísa Cortes
2,3,6
,
Luís Pereira de Almeida*
1,2,4,5
,
Rui Jorge Nobre*
1,2,3,4
1
CNC-UC – Center for Neuroscience and Cell Biology
,
University of Coimbra
,
2
CIBB – Center for Innovative Biomedicine and Biotechnology
,
University of Coimbra
,
3
IIIUC – Institute for Interdisciplinary Research
,
University of Coimbra
,
4
ViraVector – Viral Vectors for Gene Transfer Core Facility
,
University of Coimbra
,
5
FFUC – Faculty of Pharmacy
,
University of Coimbra
,
6
MICC-CNC – Microscopy Imaging Center of Coimbra – CNC
,
University of Coimbra
Tags
Adeno-associated Viral Vectors
AAV Purification
Heparin Affinity Chromatography
Transient Transfection
Density Gradient Ultracentrifugation
Scalable Production
Semi-automated Protocol
Mosaic AAV
Preclinical Studies
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