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Differenziamento e caratterizzazione di osteoclasti da cellule staminali pluripotenti indotte umane
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal Developmental Biology
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
DOI:

10:52 min

March 22, 2024

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Chapters

  • 00:03Introduction
  • 01:16Passaging iPSCs
  • 03:32Embryoid Body Induction
  • 06:10Hematopoietic Differentiation
  • 08:31M-CSF Maturation and Osteoclast Differentiation
  • 09:25Representative Results
  • 10:19Conclusion

Summary

Automatic Translation

Questo protocollo presenta il differenziamento degli osteoclasti umani dalle cellule staminali pluripotenti indotte (iPSC) e descrive i metodi per la caratterizzazione degli osteoclasti e dei precursori degli osteoclasti.

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