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Arricchimento di vescicole extracellulari native e ricombinanti di micobatteri
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06:38 min
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December 08, 2023
DOI:
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute,NCR Biotech Science Cluster, 2Clinical Microbiology Division,CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute,Rutgers University
Summary
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Questo protocollo descrive in dettaglio l'arricchimento di vescicole extracellulari micobatteriche native (mEV) da colture axeniche di Mycobacterium smegmatis (Msm) e come possono essere progettate e arricchite le MsmEV ricombinanti contenenti mCherry (un reporter fluorescente rosso). Infine, verifica il nuovo approccio con l'arricchimento di MsmEVs contenenti la proteina EsxA di Mycobacterium tuberculosis.
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Cite this Article
Jayaswal, P., Ilyas, M., Singh, K., Kumar, S., Sisodiya, L., Jain, S., Mahlawat, R., Sharma, N., Gupta, V., Atmakuri, K. Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria. J. Vis. Exp. (202), e65138, doi:10.3791/65138 (2023).
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