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ヒト人工多能性幹細胞の成熟免疫表現型を有する脳微小血管内皮細胞様細胞への分化
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
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ヒト人工多能性幹細胞の成熟免疫表現型を有する脳微小血管内皮細胞様細胞への分化

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1,597 Views

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09:03 min

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May 19, 2023

DOI:

10.3791/65134-v

DOI:
10.3791/65134-v

•

09:03 min
May 19, 2023

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6 Views
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1Graduate School of Medicine,Yamaguchi University, 2Theodor Kocher Institute,University of Bern, 3Department of Neurotherapeutics,Yamaguchi University of Medicine

Summary

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ここでは、多能性幹細胞を脳微小血管内皮細胞(BMEC)様細胞に分化させるプロトコルである拡張内皮細胞培養法(EECM)について述べる。これらの細胞は内皮細胞接着分子発現を示すため、in vitroで免疫細胞相互作用を研究するのに適したヒト血液脳関門モデルです。

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Cite this Article

Matsuo, K., Engelhardt, B., Nishihara, H. Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype. J. Vis. Exp. (195), e65134, doi:10.3791/65134 (2023).

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