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Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
JoVE Journal
生物工学
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JoVE Journal 生物工学
Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

1,583 Views

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09:03 min

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May 19, 2023

DOI:

10.3791/65134-v

DOI:
10.3791/65134-v

•

09:03 min
May 19, 2023

•

6 Views
, ,
1Graduate School of Medicine,Yamaguchi University, 2Theodor Kocher Institute,University of Bern, 3Department of Neurotherapeutics,Yamaguchi University of Medicine

概要

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Here, we describe a protocol, the extended endothelial cell culture method (EECM), that allows differentiation of pluripotent stem cells to brain microvascular endothelial cell (BMEC)-like cells. These cells show endothelial cell adhesion molecule expression and are thus a human blood-brain barrier model suitable to study immune cell interactions in vitro.

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Cite this Article

Matsuo, K., Engelhardt, B., Nishihara, H. Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype. J. Vis. Exp. (195), e65134, doi:10.3791/65134 (2023).

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