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使用
体内
显微注射和电穿孔的体细胞基因组工程小鼠模型
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Somatic Genome-Engineered Mouse Models Using
In Vivo
Microinjection and Electroporation
Please note that all translations are automatically generated.
Click here for the English version.
使用
体内
显微注射和电穿孔的体细胞基因组工程小鼠模型
DOI:
10.3791/65131-v
•
08:06 min
•
May 05, 2023
•
Keerthana Harwalkar
2
,
Nobuko Yamanaka
3
,
Yojiro Yamanaka
2,3
1
Rosalind and Morris Goodman Cancer Research Institute
,
McGill University
,
2
Department of Human Genetics
,
McGill University
,
3
McGill Integrated Core for Animal Modeling (MICAM)
,
McGill University
Chapters
00:05
Introduction
01:01
Preparing Microinjection Needle, Surgery Area, Solution, and Needle for Injection
02:26
Exposing the Female Reproductive Tract
04:11
In Vivo Oviduct Injection and Electroporation
06:29
Results: Validation of Successful In Vivo Injection and Electroporation of Oviduct Epithelial Cells
07:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了显微注射和
体内
电穿孔,用于小鼠输卵管中区域限制的CRISPR介导的基因组编辑。
Tags
Somatic Genome Engineering
Mouse Models
In Vivo Microinjection
Electroporation
Organ Targeting
Cancer Initiation
Genome Manipulation
Immunocompetent Environment
Adaptable Mutation Combinations
Oviduct Targeting
Organ Parenchyma Targeting
Glass Capillary Microinjection
Anesthesia
Aseptic Technique
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