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Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation
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Investigación sobre el cáncer
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JoVE Revista Investigación sobre el cáncer
Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

1,141 Views

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08:06 min

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May 05, 2023

DOI:

10.3791/65131-v

DOI:
10.3791/65131-v

•

08:06 min
May 05, 2023

•

15 Views
, ,
1Rosalind and Morris Goodman Cancer Research Institute,McGill University, 2Department of Human Genetics,McGill University, 3McGill Integrated Core for Animal Modeling (MICAM),McGill University

Summary

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This protocol describes microinjection and in vivo electroporation for regionally restricted CRISPR-mediated genome editing in the mouse oviduct.

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Somatic Genome EngineeringMouse ModelsIn Vivo MicroinjectionElectroporationOrgan TargetingCancer InitiationGenome ManipulationImmunocompetent EnvironmentAdaptable Mutation CombinationsOviduct TargetingOrgan Parenchyma TargetingGlass Capillary MicroinjectionAnesthesiaAseptic Technique

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Harwalkar, K., Yamanaka, N., Yamanaka, Y. Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation. J. Vis. Exp. (195), e65131, doi:10.3791/65131 (2023).

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