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Neuromodulación ultrasónica focalizada de cultivos neuronales humanos in vitro en matrices de microelectrodos multipocillos
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May 03, 2024
DOI:
1Electrical and Computer Engineering,Johns Hopkins University, 2HEPIUS Innovation Laboratory,Johns Hopkins University School of Medicine, 3Neurosurgery,Johns Hopkins University School of Medicine, 4Biomedical Engineering,Johns Hopkins University School of Medicine, 5Mechanical Engineering,Johns Hopkins University, 6Anesthesiology and Critical Care Medicine,Johns Hopkins University School of Medicine
Summary
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Aquí, presentamos un protocolo para el uso de un sistema de alto rendimiento que permite el monitoreo y la cuantificación de los efectos neuromoduladores de los ultrasonidos focalizados en neuronas de células madre pluripotentes inducidas por humanos (HiPSC).
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Cite this Article
Liang, R., Mess, G., Punnoose, J., Kempski Leadingham, K. M., Smit, C., Thakor, N., Habela, C. W., Tyler, B., Salimpour, Y., Manbachi, A. Focused Ultrasound Neuromodulation of Human In Vitro Neural Cultures in Multi-Well Microelectrode Arrays. J. Vis. Exp. (207), e65115, doi:10.3791/65115 (2024).
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