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Análise Fenotípica Semi-Automatizada de Culturas Funcionais de Células Esferoides 3D
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Análise Fenotípica Semi-Automatizada de Culturas Funcionais de Células Esferoides 3D

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734 Views

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06:48 min

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August 18, 2023

DOI:

10.3791/65086-v

DOI:
10.3791/65086-v

•

06:48 min
August 18, 2023

•

6 Views
, , , , ,
1Department of Biochemistry,Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology,University of Southern Denmark, 3CelVivo ApS

Summary

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Apresentamos um protocolo para o cultivo de esferoides de alta reprodutibilidade e sua caracterização fenotípica usando captura de imagens e proteômica.

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Cite this Article

Stransky, S., Young, D., Mikkelsen, K., Thulesen, A. P., Frandsen, H. S., Sidoli, S. Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures. J. Vis. Exp. (198), e65086, doi:10.3791/65086 (2023).

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