Journal
/
Biochemistry
/
Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

English

Automatically Generated

772 Views

•

06:48 min

•

August 18, 2023

DOI:

10.3791/65086-v

DOI:
10.3791/65086-v

•

06:48 min
August 18, 2023

•

6 Views
, , , , ,
1Department of Biochemistry,Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology,University of Southern Denmark, 3CelVivo ApS

Summary

Automatically generated

We present a protocol for growing high-reproducible spheroids and their phenotypic characterization using image capture and proteomics.

Explore More Videos

3D Cell CultureSpheroidHepG2/C3ATHLE-3Ultra-low Attachment PlateBioreactorProteomic AnalysisLC-MSPhenotypic Analysis

Read Article

Cite this Article

Stransky, S., Young, D., Mikkelsen, K., Thulesen, A. P., Frandsen, H. S., Sidoli, S. Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures. J. Vis. Exp. (198), e65086, doi:10.3791/65086 (2023).

Download .ris file

Copy

Share Video

.

Copy