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Kwantificering en karakterisering van het hele genoom van SARS-CoV-2-RNA in afvalwater- en luchtmonsters
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JoVE Journal Environment
Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
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Kwantificering en karakterisering van het hele genoom van SARS-CoV-2-RNA in afvalwater- en luchtmonsters

Nederlands

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899 Views

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09:26 min

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June 30, 2023

DOI:

10.3791/65053-v

DOI:
10.3791/65053-v

•

09:26 min
June 30, 2023

•

12 Views
, , , , , , , , ,
1Institute of Sustainable Processes,University of Valladolid, 2Department of Chemical Engineering and Environmental Technology,University of Valladolid, 3Department of Public Health Microbiology,National Laboratory of Health, Environment and Food, 4ICBAS – School of Medicine and Biomedical Sciences,Porto University, 5Epidemiology Research Unit (EPIUnit),Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)

Summary

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Dit protocol heeft tot doel SARS-CoV-2-RNA in afvalwater- en luchtmonsters te kwantificeren voor gebruik voor epidemiologische studies op basis van afvalwater en om het blootstellingsrisico aan SARS-CoV-2 in aerosolen binnen en buiten te beoordelen. Dit protocol beschrijft ook een betegelde amplicon long-template sequencing-benadering voor de karakterisering van het hele genoom van SARS-CoV-2.

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Cite this Article

Gonçalves, J., Gomes da Silva, P., Koritnik, T., Bosilj, M., Torres-Franco, A., Diaz, I., Rodriguéz, E., Marcos, E., Mesquita, J. R., García-Encina, P. Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples. J. Vis. Exp. (196), e65053, doi:10.3791/65053 (2023).

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