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Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
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09:26 min
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June 30, 2023
DOI:
1Institute of Sustainable Processes,University of Valladolid, 2Department of Chemical Engineering and Environmental Technology,University of Valladolid, 3Department of Public Health Microbiology,National Laboratory of Health, Environment and Food, 4ICBAS – School of Medicine and Biomedical Sciences,Porto University, 5Epidemiology Research Unit (EPIUnit),Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)
Summary
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This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.
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Gonçalves, J., Gomes da Silva, P., Koritnik, T., Bosilj, M., Torres-Franco, A., Diaz, I., Rodriguéz, E., Marcos, E., Mesquita, J. R., García-Encina, P. Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples. J. Vis. Exp. (196), e65053, doi:10.3791/65053 (2023).
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