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Immunology and Infection

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ラットモデルにおける虚血性プレコンディショニング効果を研究するための表在性下上腹部動脈軸フラップ

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model

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ラットモデルにおける虚血性プレコンディショニング効果を研究するための表在性下上腹部動脈軸フラップ

1,315 Views

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09:07 min

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January 27, 2023

DOI:

10.3791/64980-v

DOI:

10.3791/64980-v

•

09:07 min

January 27, 2023

•

5 Views

Yanis Berkane*^1,2,3,5, Austin Alana Shamlou*^1,2,4,5, Jose Reyes^1,2, Hyshem H. Lancia^1,2,5, Irina Filz von Reiterdank^1,2,5,6,7, Nicolas Bertheuil³, Basak E. Uygun^2,5,6, Korkut Uygun^1,2,5,6, William G. Austen Jr.^2,4, Curtis L. Cetrulo Jr.^1,2,4,5, Mark A. Randolph^1,2,4,5, Alexandre G. Lellouch^1,2,5,6,8

¹Vascularized Composite Allotransplantation Laboratory,Massachusetts General Hospital, ²Harvard Medical School, ³Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes),University of Rennes 1, ⁴Plastic Surgery Research Laboratory,Massachusetts General Hospital, ⁵Shriners Children’s Boston, ⁶Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, ⁷Department of Plastic Surgery and Hand Surgery,University Medical Center Utrecht, ⁸Department of Plastic, Reconstructive, and Aesthetic Surgery, Groupe Almaviva Santé,Clinique de l’Alma

Summary

このプロトコルでは、ラットの皮膚筋膜の採取、縫合、およびモニタリングについて説明しており、大腿血管をクランプして結紮することにより、表在性下上腹部血管を通る血流の良好な視覚化と操作を可能にします。これは、虚血性プレコンディショニングを含む研究にとって重要です。

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