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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
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Biochemistry
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JoVE Journal Biochemistry
“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome
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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

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1,529 Views

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06:31 min

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March 24, 2023

DOI:

10.3791/64952-v

DOI:
10.3791/64952-v

•

06:31 min
March 24, 2023

•

13 Views
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1Department of Laboratory Medicine,University of California, San Francisco, 2Department of Biosciences and Bioengineering,Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences,University of California, San Francisco, 4Chan Zuckerberg Biohub

Summary

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Here, we describe a proteomics workflow for characterization of the cell surface proteome of various cell types. This workflow includes cell surface protein enrichment, subsequent sample preparation, analysis using an LC-MS/MS platform, and data processing with specialized software.

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Cite this Article

Naik, A., Srivastava, S., Wiita, A. P. "Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome. J. Vis. Exp. (193), e64952, doi:10.3791/64952 (2023).

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