Journal
/
Bioquímica
/
“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome

“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome

1,579 Views

•

06:31 min

•

March 24, 2023

DOI:

10.3791/64952-v

DOI:
10.3791/64952-v

•

06:31 min
March 24, 2023

•

13 Views
, ,
1Department of Laboratory Medicine,University of California, San Francisco, 2Department of Biosciences and Bioengineering,Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences,University of California, San Francisco, 4Chan Zuckerberg Biohub

Summary

Automatically generated

Here, we describe a proteomics workflow for characterization of the cell surface proteome of various cell types. This workflow includes cell surface protein enrichment, subsequent sample preparation, analysis using an LC-MS/MS platform, and data processing with specialized software.

Explore More Videos

Cell Surface ProteomeCell Surface CaptureBiotin LabelingNeutrAvidin EnrichmentMass SpectrometryCancer ImmunotherapyCell Surface Antigens

Read Article

Cite this Article

Naik, A., Srivastava, S., Wiita, A. P. "Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome. J. Vis. Exp. (193), e64952, doi:10.3791/64952 (2023).

Download .ris file

Copy

Share Video

.

Copy