Journal
/
Biochemistry
/
“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome

“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome

DOI:
10.3791/64952-v

•

06:31 min

•

March 24, 2023

•

, ,
1Department of Laboratory Medicine,University of California, San Francisco, 2Department of Biosciences and Bioengineering,Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences,University of California, San Francisco, 4Chan Zuckerberg Biohub

Summary

Automatic Translation

Automatic Translation

Here, we describe a proteomics workflow for characterization of the cell surface proteome of various cell types. This workflow includes cell surface protein enrichment, subsequent sample preparation, analysis using an LC-MS/MS platform, and data processing with specialized software.

Tags

Cell Surface ProteomeCell Surface CaptureBiotin LabelingNeutrAvidin EnrichmentMass SpectrometryCancer ImmunotherapyCell Surface Antigens

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Read Article