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通过转录组学了解温带噬菌体对其溶原的影响
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
Please note that all translations are automatically generated.
Click here for the English version.
通过转录组学了解温带噬菌体对其溶原的影响
DOI:
10.3791/64945-v
•
09:23 min
•
January 05, 2024
•
Revathy Krishnamurthi
,
Enrique González-Tortuero
,
Grace Plahe
,
Ian B. Goodhead
,
Joanne L. Fothergill
,
Chloë E. James
,
Heather E. Allison
1
Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences (IVES)
,
University of Liverpool
,
2
School of Science, Engineering, and Environment
,
University of Salford
Chapters
00:04
Introduction
01:20
Temporal Direct Enumeration of Spontaneous Induction
02:56
Preparation of Un‐Induced and Induced Lysogen Cultures for RNA Extraction
05:00
Standard Curve and Quantitative‐PCR
06:42
Results: Understanding the Impact of Prophages on Their Hosts Using PFU and RT‐qPCR
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议能够揭示噬菌体对其宿主的影响。细菌培养物使用最能支持溶原状态的条件进行同步,从而限制了自发诱导。RT-qPCR明确区分噬菌体限制性基因和与噬菌体对照未偶联的基因与裂解复制周期中表达的基因。
Tags
Temperate Bacteriophages
Prophages
Transcriptomic Approach
Bacterial Hosts
Lysogen
Lytic Cycle
Gene Expression
Bacterial Pathogens
Therapeutic Targets
RNA Integrity
Indicator Host
Lysogens
Infective Phage Particles
Rifampicin Resistant
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