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Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
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Biologia
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JoVE Journal Biologia
Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

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09:23 min

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January 05, 2024

DOI:

10.3791/64945-v

DOI:
10.3791/64945-v

•

09:23 min
January 05, 2024

•

28 Views
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1Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences (IVES),University of Liverpool, 2School of Science, Engineering, and Environment,University of Salford

Summary

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This protocol enables the impact of prophages on their hosts to be revealed. Bacterial cultures are synchronized using conditions that best support the lysogenic state, limiting spontaneous induction. RT-qPCR unequivocally distinguishes prophage-restricted genes and those uncoupled from phage control from those that are expressed during the lytic replication cycle.

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Temperate BacteriophagesProphagesTranscriptomic ApproachBacterial HostsLysogenLytic CycleGene ExpressionBacterial PathogensTherapeutic TargetsRNA IntegrityIndicator HostLysogensInfective Phage ParticlesRifampicin ResistantTop Agar Layer

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Cite this Article

Krishnamurthi, R., González-Tortuero, E., Plahe, G., Goodhead, I. B., Fothergill, J. L., James, C. E., Allison, H. E. Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics. J. Vis. Exp. (203), e64945, doi:10.3791/64945 (2024).

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