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February 10, 2023
DOI:
1Laboratory of Molecular Immunology,The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Retrovirology,The Rockefeller University, 4Howard Hughes Medical Institute,The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine,King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center,Wistar Institute
Summary
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Wir stellen eine Methode zur Kultivierung und Gen-Editierung von primären Rhesusaffen-B-Zellen unter Verwendung von CRISPR/Cas9 und rekombinantem Adeno-assoziiertem Virus-Serotyp 6 für die Untersuchung von B-Zell-Therapien vor.
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Hartweger, H., Gautam, R., Nishimura, Y., Schmidt, F., Yao, K., Escolano, A., Jankovic, M., Martin, M. A., Nussenzweig, M. C. Gene Editing of Primary Rhesus Macaque B Cells. J. Vis. Exp. (192), e64858, doi:10.3791/64858 (2023).
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