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Überwachung von On-Target-Signalantworten in Zebrafischlarven - Z-REX entschlüsselt präzise Mechanismen elektrophiler Wirkstoffe und Metaboliten
JoVE Journal
Biology
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JoVE Journal Biology
Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites
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Überwachung von On-Target-Signalantworten in Zebrafischlarven - Z-REX entschlüsselt präzise Mechanismen elektrophiler Wirkstoffe und Metaboliten

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574 Views

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05:28 min

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June 02, 2023

DOI:

10.3791/64846-v

DOI:
10.3791/64846-v

•

05:28 min
June 02, 2023

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6 Views
, , , , ,
1Swiss Federal Institute of Technology Lausanne (EPFL), 2Division of Genetics, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Pharmacology and Toxicology, College of Pharmacy,University of Utah, 4University of Lausanne (UNIL)

Summary

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Zebrafish targeting reactive electrophiles and oxidants (Z-REX) ist eine auf chemischer Biologie basierende Methode zur Untersuchung reaktiver niedermolekularer Signalwege. Diese Technik kann auf lebende Fische in verschiedenen Entwicklungsstadien angewendet werden. Hier koppeln wir Standard-Assays im Zebrafisch mit Z-REX für die Analyse des Signalwegs.

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Cite this Article

Huang, K., Ly, P., Poganik, J. R., Parvez, S., Long, M. J. C., Aye, Y. Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites. J. Vis. Exp. (196), e64846, doi:10.3791/64846 (2023).

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