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Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

DOI:
10.3791/64846-v

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05:28 min

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June 02, 2023

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, , , , ,
1Swiss Federal Institute of Technology Lausanne (EPFL), 2Division of Genetics, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Pharmacology and Toxicology, College of Pharmacy,University of Utah, 4University of Lausanne (UNIL)

Chapters

  • 00:04Introduction
  • 01:02Fish Embryo Microinjection
  • 01:45Z‐REX Treatment
  • 02:31Downstream Assay
  • 03:13Results: Analysis of Treated and Untreated Embryos
  • 04:28Conclusion

Summary

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Zebrafish targeting reactive electrophiles and oxidants (Z-REX) is a chemical biology-based method for the investigation of reactive small-molecule signaling. This technique can be applied to live fish of different developmental stages. Here, we couple standard assays in zebrafish with Z-REX for signaling pathway analysis.

Tags

Keywords: ZebrafishZ-REXSignaling ResponsesElectrophilic DrugsMetabolitesReactive LigandTarget EngagementPhenotypeNeutrophilsMacrophagesPrecision Isoform-specific Kinase InhibitorsTriple-negative Breast Cancer

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