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クニダリアンモデル系Exaiptasia diaphanaにおけるクロマチン免疫沈降
JoVE Journal
Genetics
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JoVE Journal Genetics
Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
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クニダリアンモデル系Exaiptasia diaphanaにおけるクロマチン免疫沈降

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173 Views

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11:48 min

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March 17, 2023

DOI:

10.3791/64817-v

DOI:
10.3791/64817-v

•

11:48 min
March 17, 2023

•

3 Views
, , , , ,
1Red Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST), 2KAUST Environmental Epigenetics Program (KEEP), Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)

Summary

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モデル生物 Exaiptasia diaphana のヒストンマークH3K4me3に対するクロマチン免疫沈降(X-ChIP)アッセイが提示されます。アッセイの特異性と有効性は、定量的PCRと次世代シーケンシングによって確認されます。このプロトコルにより、イソギンチャク E.diaphanaのタンパク質-DNA相互作用の研究を増やすことができます。

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Dix, M. F., Liu, P., Cui, G., Della Valle, F., Orlando, V., Aranda, M. Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana. J. Vis. Exp. (193), e64817, doi:10.3791/64817 (2023).

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