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Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
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11:48 min
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March 17, 2023
DOI:
1Red Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST), 2KAUST Environmental Epigenetics Program (KEEP), Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)
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A chromatin immunoprecipitation (X-ChIP) assay against the histone mark H3K4me3 for the model organism Exaiptasia diaphana is presented. The specificity and effectiveness of the assay are confirmed by quantitative PCR and next-generation sequencing. This protocol enables the increased investigation of protein-DNA interactions in the sea anemone E. diaphana.
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Dix, M. F., Liu, P., Cui, G., Della Valle, F., Orlando, V., Aranda, M. Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana. J. Vis. Exp. (193), e64817, doi:10.3791/64817 (2023).
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