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Biology
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激光消融和活体显微镜研究肠道重塑
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling
Please note that all translations are automatically generated.
Click here for the English version.
激光消融和活体显微镜研究肠道重塑
DOI:
10.3791/64756-v
•
00:00 min
•
June 09, 2023
•
Dimitrios Laskaris*
1,2
,
Maria Azkanaz*
1,2
,
Mijke A. de Vreij-Kruidenier
,
Doreen van Rijswoud-Ram
,
Hendrik A. Messal
2
,
Jacco van Rheenen
2
1
Department of Molecular Pathology
,
The Netherlands Cancer Institute
,
2
Oncode Institute
,
3
Animal Laboratory Facility
,
The Netherlands Cancer Institute
Chapters
00:06
Introduction
01:04
Preparation for Surgery and Intravital Imaging
02:16
Surgery and Intravital Imaging
07:47
Repetitive Surgery and Intravital Imaging
08:17
Representative Results
09:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一种在激光诱导的伤口时获得肠道图像的方法。通过将小鼠肠道暴露于多光子激光下,局部诱导单个或多个隐窝的丢失。通过在几个月内对受损区域进行重复成像,可以捕获肠道恢复的实时动态。
Tags
Laser Ablation
Intravital Microscopy
Intestinal Remodeling
Injury Models
Regeneration
Intestinal Epithelium
Crypt Dynamics
Two-photon Microscopy
Anesthesia
Temperature Control
Surgical Procedures
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