Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Laser Ablation and Intravital Microscopy to Study …
JoVE Journal
Biology
Author Produced

Esto contenido es Free Access.

JoVE Journal Biology
Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling
 

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Article DOI: 10.3791/64756-v • June 9th, 2023
June 9th, 2023 •
*1,2, *1,2, 3, 3, 1,2, 1,2
1Department of Molecular Pathology, The Netherlands Cancer Institute, 2Oncode Institute, 3Animal Laboratory Facility, The Netherlands Cancer Institute
* These authors contributed equally

Capítulos

Resumen
Automatic Translation

Here, we present a method to obtain images of the intestine upon laser-induced wounding. By exposing the mouse intestine to a multiphoton laser, the loss of a single or multiple crypt(s) is induced locally. By repeatedly imaging the damaged area over months, the real-time dynamics of intestinal recovery is captured.

Tags

Laser Ablation Intravital Microscopy Intestinal Remodeling Injury Models Regeneration Longitudinal Imaging Protocols Cell And Tissue Scale Dynamics Intestinal Epithelium Photon-based Laser Ablation Single Crypt Larger Intestinal Fields Time And Space Control Intravital Imaging Crypt Dynamics Tissue Recovery Tamoxifen Injection Surgery Preparation Analgesia Administration
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter