The presented protocol describes a straightforward approach for screening protein crystallization conditions and crystal growth using a 96-well high-throughput dialysis plate. The use of dialyzer tubes for the large-scale growth of microcrystals is also demonstrated for serial crystallography and MicroED applications.
Kwan, T. O. C., Danson, A. E., Draper, P., Reardon, P., Moraes, I. High-Throughput Protein Crystallization via Microdialysis. J. Vis. Exp. (193), e64744, doi:10.3791/64744 (2023).