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High-Throughput Protein Crystallization via Microdialysis
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JoVE Journal Biyokimya
High-Throughput Protein Crystallization via Microdialysis

High-Throughput Protein Crystallization via Microdialysis

DOI:
10.3791/64744-v

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06:18 min

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March 03, 2023

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, , , ,
1National Physical Laboratory, 2SWISSCI LTD

Bölümler

  • 00:04Introduction
  • 00:44Setting up the Microdialysis Plate
  • 02:37Large-Scale Crystallization Using Dialyzer Tubes
  • 03:52Results: Protein Microcrystal Formation in Microdialysis Plates and Dialyzer Tubes
  • 05:46Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The presented protocol describes a straightforward approach for screening protein crystallization conditions and crystal growth using a 96-well high-throughput dialysis plate. The use of dialyzer tubes for the large-scale growth of microcrystals is also demonstrated for serial crystallography and MicroED applications.

Etiketler

Protein CrystallizationMicrodialysisHigh-throughputDialysis96-well PlateCrystal GrowthLow-volume PipettingMultichannel PipetteUV Cover FilmDialysis SolutionTemperature-controlled IncubatorLarge-scale Crystallization

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