Journal
/
Immunology and Infection
/
Detección de células T polifuncionales en niños vacunados con la vacuna contra la encefalitis japonesa mediante la técnica de citometría de flujo
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
Please note that all translations are automatically generated. Click here for the English version.

Detección de células T polifuncionales en niños vacunados con la vacuna contra la encefalitis japonesa mediante la técnica de citometría de flujo

español

Automatically Generated

1,714 Views

•

09:37 min

•

September 23, 2022

DOI:

10.3791/64671-v

DOI:
10.3791/64671-v

•

09:37 min
September 23, 2022

•

3 Views
, , , , , , ,
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children,2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine,Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing),Becton Dickinson Medical Devices (Shanghai) Co Ltd

Summary

Automatically generated

El presente protocolo combina la estimulación ex vivo y la citometría de flujo para analizar los perfiles de células T polifuncionales (T PF) en células mononucleares de sangre periférica (PBMC) en niños vacunados contra el virus de la encefalitis japonesa (JEV). El método de detección y el esquema de color de citometría de flujo de los TPF específicos de JEV se probaron para proporcionar una referencia para estudios similares.

Explore More Videos

Japanese Encephalitis VaccinePolyfunctional T CellsFlow CytometryPBMC StimulationCell Surface Marker StainingIntracellular Cytokine StainingVirus-specific T Cells

Read Article

Cite this Article

Zhang, L., Zhang, M., Liu, M., Ai, J., Tian, J., Ge, H., Wang, R., Xie, Z. Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique. J. Vis. Exp. (187), e64671, doi:10.3791/64671 (2022).

Download .ris file

Copy

Share Video

.

Copy