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Detecção de Células T Polifuncionais em Crianças Vacinadas com Encefalite Japonesa através da Técnica de Citometria de Fluxo
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09:37 min
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September 23, 2022
DOI:
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children,2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine,Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing),Becton Dickinson Medical Devices (Shanghai) Co Ltd
Summary
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O presente protocolo combina estimulação ex vivo e citometria de fluxo para analisar perfis polifuncionais de células T (TPF) em células mononucleares do sangue periférico (PBMCs) em crianças vacinadas com o vírus da encefalite japonesa (JEV). O método de detecção e o esquema de cores da citometria de fluxo de TPFs específicos do JEV foram testados para fornecer uma referência para estudos semelhantes.
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Zhang, L., Zhang, M., Liu, M., Ai, J., Tian, J., Ge, H., Wang, R., Xie, Z. Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique. J. Vis. Exp. (187), e64671, doi:10.3791/64671 (2022).
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