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使用改良的夹心 ELISA 定量来自中性粒细胞胞外陷阱的髓过氧化物酶 DNA 和中性粒细胞弹性蛋白酶 DNA 复合物

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Immunology and Infection

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JoVE Journal Immunology and Infection

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Please note that all translations are automatically generated. Click here for the English version.

使用改良的夹心 ELISA 定量来自中性粒细胞胞外陷阱的髓过氧化物酶 DNA 和中性粒细胞弹性蛋白酶 DNA 复合物

DOI:

10.3791/64644-v

•

07:18 min

•

May 12, 2023

•

Md. Monirul Islam, Umme Salma, Takayuki Irahara, Eizo Watanabe, Naoshi Takeyama

¹Department of Biochemistry and Biotechnology,University of Science and Technology Chittagong (USTC), ²Department of Emergency and Critical Care Medicine,Aichi Medical University

Chapters

00:04Introduction
01:18Sample Collection and Storage
02:13Modified Sandwich Enzyme‐Linked Immunosorbent Assay
05:42Results: Myeloperoxidase‐DNA (MPO‐DNA) and Neutrophil Elastase‐DNA (NE‐DNA) Complex Quantification
06:54Conclusion

Summary

Automatic Translation

我们提出了一种改进的夹心酶联免疫吸附测定技术方案，以定量测量中性粒细胞细胞外捕获残留物的两种成分，髓过氧化物酶偶联-DNA 和中性粒细胞弹性蛋白酶偶联 DNA 复合物，来源于活化的中性粒细胞。

Tags

Keywords: Myeloperoxidase-DNA Neutrophil Elastase-DNA NETs ELISA Sepsis ARDS Damage-associated Molecular Patterns Polymorphonuclear Neutrophils PMA DNase I EDTA

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