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A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
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JoVE Journal 発生生物学
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

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08:19 min

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December 16, 2022

DOI:

10.3791/64582-v

DOI:
10.3791/64582-v

•

08:19 min
December 16, 2022

•

1 Views
, , , , , , , ,
1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Département de Biologie,École Normale Supérieure de Lyon, 4Department of Critical Care Medicine,University of Pittsburgh

概要

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This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.

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Fetal MouseCongenital Heart DefectsEchocardiographyNecropsyConfocal MicroscopyAnatomical ChangesStructural Birth DefectsUltrasoundColor DopplerSample PreparationLaser Confocal ImagingImage Acquisition

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Cite this Article

Guzman-Moreno, C., Zhang, P., Phillips, O. R., Block, M., Glennon, B. J., Holbrook, M., Weigand, L., Lo, C. W., Lin, J. I. A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse. J. Vis. Exp. (190), e64582, doi:10.3791/64582 (2022).

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