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使用微透析系统实时动态收集意识大鼠的海马细胞外液
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Real-Time Dynamic Collection of Hippocampal Extracellular Fluid from Conscious Rats Using a Microdialysis System
Please note that all translations are automatically generated.
Click here for the English version.
使用微透析系统实时动态收集意识大鼠的海马细胞外液
DOI:
10.3791/64530-v
•
05:43 min
•
October 21, 2022
•
Xiaobo Wang
2
,
Na Xie
,
Yi Zhang
,
Xianli Meng
2,3
,
Ya Hou
,
Sanyin Zhang
2
1
Innovative Institute of Chinese Medicine and Pharmacy
,
Chengdu University of Traditional Chinese Medicine
,
2
Research Institute of Integrated TCM & Western Medicine
,
Chengdu University of Traditional Chinese Medicine
,
3
School of Pharmacy
,
Chengdu University of Traditional Chinese Medicine
,
4
School of Ethnic Medicine
,
Chengdu University of Traditional Chinese Medicine
Chapters
00:04
Introduction
00:44
Brain Microdialysis Probe Implantation Surgery
02:24
Microdialysis System Connection and Probe Check
03:15
Collection of Hippocampal Extracellular Fluid (HECF) from the Awake Rat
04:02
Maintenance of the Microdialysis System and Devices After Sampling
04:36
Results: Analysis of HECF Collected Using the Microdialysis Sampling Equipment
04:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里的协议提供了使用微透析系统从清醒大鼠海马体中细胞外液的详细实时动态采样。
Tags
Hippocampal Extracellular Fluid
Brain Microdialysis
Real-time Dynamic Collection
Conscious Rats
Central Nervous Diseases
Stereotaxic Brain Locator
Cranial Drill
Microdialysis Casing
ACSF
Multi-channel Swivel Controller
Microsyringe Pump
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