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Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography
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September 05, 2012
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A method to determine the time course of ethanol concentration in the brains of rats during operant ethanol self-administration is described. Gas chromatography with flame ionization detection is used to quantify ethanol in the dialysate samples, because it has the sensitivity required for the small volumes that are generated.
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MicrodialysisEthanolOperant Self-administrationGas ChromatographyPharmacological EffectsDrugs Of AbuseOrally Administered DrugsIntravenously Administered DrugsPharmacokineticsBrain Dialysate Ethanol ConcentrationsMicrodialysis-behavioral ExperimentsStereotaxic SurgerySample CollectionDialysate Ethanol AnalysisGas Chromatography With Flame-ionization Detection
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Schier, C. J., Mangieri, R. A., Dilly, G. A., Gonzales, R. A. Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography. J. Vis. Exp. (67), e4142, doi:10.3791/4142 (2012).
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