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Microscopia de super-resolução do complexo sinaptonemal dentro da linha germinal de Caenorhabditis elegans
JoVE Journal
Biology
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JoVE Journal Biology
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Microscopia de super-resolução do complexo sinaptonemal dentro da linha germinal de Caenorhabditis elegans

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2,191 Views

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09:14 min

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September 13, 2022

DOI:

10.3791/64363-v

DOI:
10.3791/64363-v

•

09:14 min
September 13, 2022

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6 Views
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1Cell Biology and Biophysics,European Molecular Biology Laboratory, 2Collaboration for joint PhD degree between EMBL and Heidelberg University,Faculty of Biosciences, 3EMBL Imaging Centre,European Molecular Biology Laboratory

Summary

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A microscopia de super-resolução pode fornecer uma visão detalhada da organização dos componentes dentro do complexo sinaptonemal na meiose. Aqui, demonstramos um protocolo para resolver proteínas individuais do complexo sinaptonemal de Caenorhabditis elegans .

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Cite this Article

Čavka, I., Power, R. M., Walsh, D., Zimmermann, T., Köhler, S. Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline. J. Vis. Exp. (187), e64363, doi:10.3791/64363 (2022).

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