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Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
JoVE Journal
Biologia
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JoVE Journal Biologia
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

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09:14 min

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September 13, 2022

DOI:

10.3791/64363-v

DOI:
10.3791/64363-v

•

09:14 min
September 13, 2022

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6 Views
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1Cell Biology and Biophysics,European Molecular Biology Laboratory, 2Collaboration for joint PhD degree between EMBL and Heidelberg University,Faculty of Biosciences, 3EMBL Imaging Centre,European Molecular Biology Laboratory

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Super-resolution microscopy can provide a detailed insight into the organization of components within the synaptonemal complex in meiosis. Here, we demonstrate a protocol to resolve individual proteins of the Caenorhabditis elegans synaptonemal complex.

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Super-resolution MicroscopySynaptonemal ComplexCaenorhabditis ElegansGermlineMeiosisImmunostainingSingle Molecule Localization MicroscopyDissectionFixationAntibody Staining

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Čavka, I., Power, R. M., Walsh, D., Zimmermann, T., Köhler, S. Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline. J. Vis. Exp. (187), e64363, doi:10.3791/64363 (2022).

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