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ヒト細胞におけるHSV-1感染後のRIPK3およびMLKLのZBP1依存性リン酸化の免疫蛍光染色のためのチラミドシグナル増幅
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Immunology and Infection
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JoVE Journal Immunology and Infection
Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
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ヒト細胞におけるHSV-1感染後のRIPK3およびMLKLのZBP1依存性リン酸化の免疫蛍光染色のためのチラミドシグナル増幅

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1,902 Views

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09:15 min

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October 20, 2022

DOI:

10.3791/64332-v

DOI:
10.3791/64332-v

•

09:15 min
October 20, 2022

•

4 Views
, , ,
1VIB-UGent Center for Inflammation Research, 2Department of Biomedical Molecular Biology,Ghent University

Summary

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免疫蛍光染色中のチラミドシグナル増幅により、HSV-1感染後のZBP1誘発ネクロトーシス中のリン酸化RIPK3およびMLKLの高感度検出が可能になります。

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Tyramide Signal AmplificationImmunofluorescent StainingZBP1PhosphorylationRIPK3MLKLNecroptosisHSV-1Human CellsMicroscopyCell CultureFixationPermeabilizationAntibody Staining

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Nemegeer, J., Lemeire, K., Vandenabeele, P., Maelfait, J. Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells. J. Vis. Exp. (188), e64332, doi:10.3791/64332 (2022).

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