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Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
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JoVE Journal 免疫学と感染
Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

1,902 Views

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09:15 min

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October 20, 2022

DOI:

10.3791/64332-v

DOI:
10.3791/64332-v

•

09:15 min
October 20, 2022

•

4 Views
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1VIB-UGent Center for Inflammation Research, 2Department of Biomedical Molecular Biology,Ghent University

概要

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Tyramide signal amplification during immunofluorescent staining enables the sensitive detection of phosphorylated RIPK3 and MLKL during ZBP1-induced necroptosis after HSV-1 infection.

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Tyramide Signal AmplificationImmunofluorescent StainingZBP1PhosphorylationRIPK3MLKLNecroptosisHSV-1Human CellsMicroscopyCell CultureFixationPermeabilizationAntibody Staining

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Cite this Article

Nemegeer, J., Lemeire, K., Vandenabeele, P., Maelfait, J. Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells. J. Vis. Exp. (188), e64332, doi:10.3791/64332 (2022).

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