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改进小鼠噪声性听力损失的实验条件及听力功能和外毛细胞损伤的评估
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage
Please note that all translations are automatically generated.
Click here for the English version.
改进小鼠噪声性听力损失的实验条件及听力功能和外毛细胞损伤的评估
DOI:
10.3791/64286-v
•
07:13 min
•
February 10, 2023
•
Dian-Shiue Lee
,
Yu-Ling Pan
,
Yi-Chao Hsu
2
1
Department of Audiology and Speech-Language Pathology
,
MacKay Medical College
,
2
Institute of Biomedical Sciences
,
MacKay Medical College
Chapters
00:04
Introduction
01:04
Induction of Noise‐Induced Hearing Loss (NIHL) in Mice
03:07
Auditory Brainstem Response (ABR)‐Based Assessment of Hearing Threshold
04:19
Microscope Examination
05:18
Results: Assessing Hearing Function and Outer Hair Cell Damage in NIHL Mice
06:32
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了噪声诱导听力损失(NIHL)小鼠模型的方案。为了诱导NIHL,我们开发了一种使用波纹塑料,捕鼠笼和扬声器的新型简单设备。采用听觉脑干反应和免疫荧光成像分别评估听力功能和外毛细胞损伤。
Tags
Noise-induced Hearing Loss
Mice
ABR
Outer Hair Cell Damage
Experimental Conditions
Hearing Function Assessment
Animal Model
Sound Exposure
Hearing Threshold
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