Journal
/
Developmental Biology
/
Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
JoVE Journal
Developmental Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

DOI:
10.3791/64273-v

•

07:02 min

•

July 12, 2022

•

, , ,
1Cardiovascular Regeneration Program,Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Cardiovascular Development Group, Department of Experimental Biology,University of Jaén, Jaén, 3Fundación Medina, Granada

Chapters

  • 00:04Introduction
  • 00:41Tools and Holder Customization
  • 02:29Embryo Dissection
  • 03:50Microscope Preparation, Embryo Mounting, and Image Acquisition
  • 05:56Results: 3D Live Imaging of Cardiac Progenitor Cells in the Mouse Embryo
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.

Tags

Keywords: Live ImagingEarly Cardiac ProgenitorsMouse EmbryoCardiogenesisEmbryo DevelopmentTungsten WireGlass CapillaryPolymethyl MethacrylateStereo MicroscopeUterusDeciduiEctoplacental ConeReichter’s MembraneDissection MediumCulture Medium

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64273?article_type=v

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
check_url/64273?article_type=v

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
check_url/64273?article_type=v

Imaging Subcellular Structures in the Living Zebrafish Embryo
check_url/64273?article_type=v

Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
check_url/64273?article_type=v

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
check_url/64273?article_type=v

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
check_url/64273?article_type=v

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
check_url/64273?article_type=v

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
check_url/64273?article_type=v

Live Imaging of Mouse Secondary Palate Fusion
check_url/64273?article_type=v

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse

Read Article