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发育生物学
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Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

DOI:
10.3791/64273-v

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07:02 min

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July 12, 2022

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, , ,
1Cardiovascular Regeneration Program,Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Cardiovascular Development Group, Department of Experimental Biology,University of Jaén, Jaén, 3Fundación Medina, Granada

Chapters

  • 00:04Introduction
  • 00:41Tools and Holder Customization
  • 02:29Embryo Dissection
  • 03:50Microscope Preparation, Embryo Mounting, and Image Acquisition
  • 05:56Results: 3D Live Imaging of Cardiac Progenitor Cells in the Mouse Embryo
  • 06:35Conclusion

Summary

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We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.

Tags

Keywords: Live ImagingEarly Cardiac ProgenitorsMouse EmbryoCardiogenesisEmbryo DevelopmentTungsten WireGlass CapillaryPolymethyl MethacrylateStereo MicroscopeUterusDeciduiEctoplacental ConeReichter’s MembraneDissection MediumCulture Medium

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