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Evaluación de la transcitosis intestinal de aislados de bacteriemia neonatal por Escherichia coli
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
DOI:

08:32 min

February 17, 2023

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Chapters

  • 00:04Introduction
  • 00:44Preparing Transcytosis Inserts with T84 Cells (Approximately 1 – 2 Weeks Before the Experiment)
  • 03:43Preparing T84 Cells 1 Day Before the Experiment Using TCM w/o Antibiotics and Escherichia coli Cultures (Started 1 Day Before the Experiment)
  • 04:19Preparing E. coli Inoculum, Epithelial Cells, and Materials (On the Morning of the Experiment)
  • 05:05Inoculation of the Cells (Start of the Experiment)
  • 05:54Quantifying the Transcytosis and End of the Experiment
  • 06:56Results: Transcytosis of Neonatal E. coli Isolates
  • 08:04Conclusion

Summary

Automatic Translation

Escherichia coli causa sepsis en neonatos que ingieren la bacteria alrededor del momento del nacimiento. El proceso involucrado en la capacidad de E. coli para viajar desde el tracto entérico al torrente sanguíneo es poco conocido. Este modelo in vitro evalúa la capacidad de las cepas de E. coli para viajar a través de las células epiteliales intestinales.

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