Escherichia coli causes sepsis in neonates who ingest the bacteria around the time of birth. The process involved in E. coli’s ability to travel from the enteric tract to the bloodstream is poorly understood. This in vitro model assesses the ability of E. coli strains to travel through the intestinal epithelial cells.
Islam, A., Wheatley, J. L., Chavez-Bueno, S. Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates. J. Vis. Exp. (192), e64241, doi:10.3791/64241 (2023).