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Evaluación y manipulación de la actividad neuronal mediante microscopía holográfica de dos fotones
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1Department of Anatomy and Molecular Cell Biology,Nagoya University Graduate School of Medicine, 2Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences,National Institutes of Natural Sciences, 3Department of System Science,Kobe University Graduate School of System Informatics, 4Department of Biology, Graduate School of Sciences,Kobe University, 5Department of Information Science,Kobe University Graduate School of System Informatics, 6Center of Optical Scattering Image Science,Kobe University
Chapters
- 00:04Introduction
- 00:55Surgery and Adeno‐Associated Virus (AAV) Injection
- 02:50Preparation for the Holographic Stimulation or Illumination System
- 04:54Ca2+ Imaging Using an Image Sensor with Holographic Illumination
- 06:06Two‐Photon Imaging with Optogenetics Using a Holographic Microscope
- 08:04Results: Imaging and Functional Connectivity Using a Holographic Microscope
- 09:26Conclusion
Desarrollamos un microscopio holográfico de dos fotones que puede visualizar, evaluar y manipular la actividad neuronal utilizando una alta resolución espaciotemporal, con el objetivo de dilucidar la patogénesis de los trastornos neuropsiquiátricos que están asociados con la actividad neuronal anormal.
