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Zygoten-Mikroinjektion zur Erzeugung von Genkassetten-, Knock-in- und Flox-Allelen in Mäusen
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
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Zygoten-Mikroinjektion zur Erzeugung von Genkassetten-, Knock-in- und Flox-Allelen in Mäusen

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3,604 Views

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08:48 min

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June 24, 2022

DOI:

10.3791/64161-v

DOI:
10.3791/64161-v

•

08:48 min
June 24, 2022

•

26 Views
, , , , , , ,
1Laboratory Animal Resource Center and Trans-Border Medical Research Center,University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba

Summary

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Das vorliegende Protokoll beschreibt die Mikroinjektion von CRISPR-Cas9 und Spender-DNA durch Zygoten, um effizient Genkassetten-, Knock-in- und Flox-Mäuse zu produzieren.

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Tanimoto, Y., Mikami, N., Ishida, M., Iki, N., Kato, K., Sugiyama, F., Takahashi, S., Mizuno, S. Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice. J. Vis. Exp. (184), e64161, doi:10.3791/64161 (2022).

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