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Micro-injection de zygote pour la création d’allèles knock-in et flox de cassette génique chez la souris
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
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Micro-injection de zygote pour la création d’allèles knock-in et flox de cassette génique chez la souris

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3,604 Views

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08:48 min

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June 24, 2022

DOI:

10.3791/64161-v

DOI:
10.3791/64161-v

•

08:48 min
June 24, 2022

•

26 Views
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1Laboratory Animal Resource Center and Trans-Border Medical Research Center,University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba

Summary

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Le présent protocole décrit la micro-injection de zygotes de CRISPR-Cas9 et d’ADN de donneur pour produire efficacement des souris frappées par cassette de gènes et floxées.

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Cite this Article

Tanimoto, Y., Mikami, N., Ishida, M., Iki, N., Kato, K., Sugiyama, F., Takahashi, S., Mizuno, S. Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice. J. Vis. Exp. (184), e64161, doi:10.3791/64161 (2022).

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