/
/
Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1,097 Views
•
07:15 min
•
July 22, 2022
DOI:
Summary
Automatically generated
In vitro transcription assays can decipher the mechanisms of transcriptional regulation in Borreliella burgdorferi. This protocol describes the steps to purify B. burgdorferi RNA polymerase and perform in vitro transcription reactions. Experimental approaches using in vitro transcription assays require reliable purification and storage of active RNA polymerase.
Read Article
Cite this Article
Boyle, W. K., Sorensen, H. N., Bourret, T. J. Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays. J. Vis. Exp. (185), e64134, doi:10.3791/64134 (2022).
Copy