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Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays
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Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays
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July 22, 2022
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Summary
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In vitro transcription assays can decipher the mechanisms of transcriptional regulation in Borreliella burgdorferi. This protocol describes the steps to purify B. burgdorferi RNA polymerase and perform in vitro transcription reactions. Experimental approaches using in vitro transcription assays require reliable purification and storage of active RNA polymerase.
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Boyle, W. K., Sorensen, H. N., Bourret, T. J. Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays. J. Vis. Exp. (185), e64134, doi:10.3791/64134 (2022).
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