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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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11:40 min

June 23, 2022

DOI:

11:40 min
June 23, 2022

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Summary

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A neuronal lysosome proximity labeling proteomics protocol is described here to characterize the dynamic lysosomal microenvironment in human induced pluripotent stem cell-derived neurons. Lysosomal membrane proteins and proteins that interact with lysosomes (stably or transiently) can be accurately quantified in this method with excellent intracellular spatial resolution in live human neurons.

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