このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
2,265 Views
•
11:40 min
•
June 23, 2022
DOI:
概要
Automatically generated
A neuronal lysosome proximity labeling proteomics protocol is described here to characterize the dynamic lysosomal microenvironment in human induced pluripotent stem cell-derived neurons. Lysosomal membrane proteins and proteins that interact with lysosomes (stably or transiently) can be accurately quantified in this method with excellent intracellular spatial resolution in live human neurons.
Read Article
Cite this Article
Frankenfield, A., Ni, J., Hao, L. Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics. J. Vis. Exp. (184), e64132, doi:10.3791/64132 (2022).
Copy